Quick-16S Plus NGS Library Prep Kit (V3-V4), 24 Rxns

Primer Coverage:bacteria (84.2%), archaea (75.8%), and eukaryota (0.0%)Required Equipment:Microcentrifuge, plate spinner (centrifuge), 96-well real-time quantitative PCR system (SYBR Green compatible) or standard PCR system, and 96-well real-time PCR plates.Sample Input:Purified microbial DNA 100 ng, free of PCR inhibitors. Real-time PCR enables absolute microbial copy number quantification.Storage Temperature: -20CStorage Conditions: FreezerAmplicon Size:The final amplicon size after 1-Step PCR (targeted amplification and barcode addition) is ~606 bp.Barcode Sequences:10 bp barcodes, Available for download here (USA Only), or under the Documents section as "Barcode Sequences". 100% automation ready with only a single PCR step and without the need for normalization. The Quick-16S Plus NGS Library Prep Kit (V3-V4) is the fastest and simplest NGS library prep targeting the V3-V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a single qPCR/PCR for combined targeted amplification and barcode addition using specially designed primers. Additional library quantification analysis such as TapeStation analysis or gel electrophoresis are not necessary. For assistance with sample sheet setup, see Appendix F.Research or further manufacturing use only, not for food or drug use. . Index Primers:Dual index (barcodes) to uniquely label samples. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.The most streamlined NGS kit with only 30 minutes of hands-on time for 96 samples. Sequencing Platform:Illumina MiSeq without the need to add custom sequencing primers. After pooling by equal volume, a single clean-up of the final library is performed, rather than massive AMPure bead-based clean-ups.

Lab Supplies & Consumables,Microbiology Media

Quick-16S Plus NGS Library Prep Kit (V3-V4), 24 Rxns