DNase I Set
About The It is typically used for selectively degrading DNA in the presence of RNA.50 x 3. It is compatible with all of our RNA kits featuring in-column DNase digestion
The features lyophilized enzyme and DNA Digestion Buffer. Included: One unit (U) is defined as the amount of enzyme required to degrade 1 µg λ DNA completely in 10 minutes at 37°C in a 50 µl reaction volume (40 mM Tris-HCl, pH 8.0, 10 mM NaCl, 6 mM MgCl2, and 10 mM CaCl2). It is compatible with all of our RNA kits featuring in-column DNase digestion. This DNase is suited for applications such as nick translation, production of random fragments, cleavage of genomic DNA for footprinting, removal of DNA template after in vitro transcription, and removal of DNA from RNA samples prior to applications such as RT-PCR. One unit of enzyme is equivalent to one Kunitz unit. DNase I (RNase-free) cuts both double-stranded and single-stranded DNA, producing 3€²-OH oligonucleotides. Lyophilized enzyme provided with DNA Digestion BufferStorage Temperature: -20CStorage Conditions: FreezerConcentration: 1 U/µl Heat inactivation: Add a final concentration of 5 mM EDTA, then incubate at 65°C for 10 min.. It is typically used for selectively degrading DNA in the presence of RNA. Resuspension Volume: 275 µlSize: 250 UShipping Weight: 0.5 lbsShipping Dimensions: 5.50 x 3.50 x 2.00Research or further manufacturing use only, not for food or drug use.
Lab Supplies & Consumables,Microbiology Media
DNase I Set
Lab Supplies & Consumables,Microbiology Media
Specifications of DNase I Set | |
|---|---|
| Category | |
| Instock | instock |
