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ZYMO RESEARCH

Methylated & Non-Methylated PUC19 DNA Set, 5 Ug

About The The set is ideal as a €œspike-in€ control to assess bisulfite conversion efficiency within the same reaction as the sample, or to produce known mixtures of methylated and non-methylated DNA for assay calibration.00Research or further manufacturing use only, not for food or drug use.30 x 3

Concentration: 1 ng/µlStorage: -20°CShipping Weight: 0.125 lbsShipping Dimensions: 5.30 x 3.30 x 1.00Research or further manufacturing use only, not for food or drug use. The set is ideal as a €œspike-in€ control to assess bisulfite conversion efficiency within the same reaction as the sample, or to produce known mixtures of methylated and non-methylated DNA for assay calibration. The Methylated & Non-methylated pUC19 DNA Set consists of control DNAs and a set of specifically designed primers. The non-methylated pUC19 DNA is pUC19 isolated from a methylation-negative strain of bacteria (Dam€“, Dcm€“), and the methylated pUC19 DNA is pUC19 enzymatically methylated at all cytosines in the dinucleotide sequence 5€²...CpG...3€² by CpG Methylase.Includes pUC19MN primers in 20 µlStorage Temperature: -20CStorage Conditions: FreezerAdditional Info: pUC19 DNA has been linearized at position 2177 using ScaI endonuclease.

Lab Supplies & Consumables,Microbiology Media

Methylated & Non-Methylated PUC19 DNA Set, 5 Ug

Lab Supplies & Consumables,Microbiology Media

Specifications of Methylated & Non-Methylated PUC19 DNA Set, 5 Ug

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Methylated & Non-Methylated PUC19 DNA Set, 5 Ug
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    • Ideal for locus specific and global quantification of hydroxymethylated DNA.Concentration: 2 U/µl Standard Reaction Time: 2 hours Storage: 5-hmC Glucosyltransferase for 12 months at -20°C.

    Ideal for locus specific and global quantification of hydroxymethylated DNA.Concentration: 2 U/µl Standard Reaction Time: 2 hours Storage: 5-hmC Glucosyltransferase for 12 months at -20°C.

    Unit Definition: One unit (U) is defined as the amount of enzyme needed to protect 1 µg of 5-hmC DNA Standard [D5405-3] from Csp6I restriction enzyme digestion via glucosylation in a reaction incubated at 30°C for 1 hour.

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    • The Methylated & Non-methylated pUC19 DNA Set consists of control DNAs and a set of specifically designed primers.

    The Methylated & Non-methylated pUC19 DNA Set consists of control DNAs and a set of specifically designed primers.

    ...Methylated & Non-Methylated pUC19 DNA Set, 5 ug Concentration: 1 ng/µlStorage: -20°CShipping Weight: 0.

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    • The Methylated & Non-methylated pUC19 DNA Set consists of control DNAs and a set of specifically designed primers.
  • Lab Supplies & Consumables,Microbiology Media
  • ZYMO RESEARCH
    • Sleek and contemporary, the Aideen collection incorporates rectangular and square shapes to achieve it’s cutting edge design.
  • Decorative Lighting,Pendants
  • Z-LITE