OneStep QMethyl-PCR Kit

Processing Time: 4.5 hoursStorage: Store at -20°C for up to 12 months. Require A260/280 g&t; 1.8, A260/230 > 1.8. Avoid repeated freeze-thaw cycles of reagents. Prolong storage at -80°C. DNA in the Test Reaction is digested with Methylation Sensitive Restriction Enzymes (MSREs) while DNA in the Reference Reaction is not. The DNA from both samples is then amplified using real-time PCR in the presence of SYTO 9 fluorescent dye and quantitated using a simple equation. Minimize light exposure.Shipping Weight: 0.125 lbsShipping Dimensions: 6.00 x 5.00 x 1.00Research or further manufacturing use only, not for food or drug use. The OneStep qMethyl Kit provides a simple, straightforward, and bisulfite-free procedure for rapid, locus-specific DNA methylation assessment via the selective amplification of a methylated region of DNA. This is accomplished by splitting any DNA to be tested into two parts: a €œTest Reaction€ and a €œReference Reaction€. 2X Test Reaction PreMix and 2X Reference Reaction PreMix are light sensitive. Single step, real-time PCR procedure for bisulfite-free determination of DNA methylation statusIncludes reagents and controls for quantitative detection and reliable performanceIdeal for rapid screening of single and multi-locus DNA methylationInput: High quality DNA suitable for use in restriction enzyme digestion.

Lab Supplies & Consumables,Microbiology Media

OneStep QMethyl-PCR Kit