RRHP 5-hmC Library Prep Kit, 25 Preps

Fragments with higher 5-hmC levels will be correlated with higher frequency of sequencing reads. Innovative library preparation for strand-specific mapping of 5-hmC in DNA.Streamlined workflow accommodates low (100ng) DNA inputs. Therefore, only fragments containing 5-hmC will be successfully amplified and analyzed by Next-Generation Sequencing. Fragments lacking glucosylated 5-hmC at the adapter-ligation junction will be cleaved and not amplified by PCR. Libraries are ready for Next-Generation sequencing (Illumina-compatible).Storage Temperature: -20CStorage Conditions: FreezerEquipment Required: Microcentrifuge, thermocycler with heated lid, and gel electrophoresis or other equipment for DNA visualizationInput: 100 ng - 1 µgSample Source: High quality, intact genomic DNA free of enzymatic inhibitors. The RRHP 5-hmC Library Prep Kit is an all-inclusive solution for analysis of genome-wide 5-hydroxymethylcytosine (5-hmC) positions at single-base resolution. RRHP bypasses the need for bisulfite conversion, which allows for DNA inputs as low as 100 ng, lower sequencing depth, and straight-forward bioinformatics processing. The Reduced Representation Hydroxymethylation Profiling (RRHP) method is based on blocking MspI digestion by glucosylating 5-hmC within MspI recognition sites. Sequencing Compatibility: Any Illumina sequencing platformsShipping Weight: 0.5 lbsShipping Dimensions: 6.00 x 5.00 x 2.00Research or further manufacturing use only, not for food or drug use.

Lab Supplies & Consumables,Microbiology Media

RRHP 5-hmC Library Prep Kit, 25 Preps